Genetic analysis of the complete gag and env genes of HIV type 1 subtype C primary isolates from South Africa

dc.contributor.authorEngelbrecht, Susan
dc.contributor.authorDe Villiers, Tania
dc.contributor.authorSampson, Candice C.
dc.contributor.authorZur Megede, Jan
dc.contributor.authorBarnett, Susan W.
dc.contributor.authorJanse van Rensburg, Estrelita
dc.date.accessioned2007-07-31T09:08:02Z
dc.date.available2007-07-31T09:08:02Z
dc.date.issued2001-11
dc.descriptionThe publisher does not allow open access to the full text of this sequence note. If you however need access to the full text, please contact the Collection Administrator, or access the item via the e-journal collection.en
dc.description.abstractSouth Africa has one of the fastest growing HIV-1 epidemics, with an estimated 4.7 million people infected. To better understand the genetic diversity of this epidemic and its potential impact on vaccine development, we have cloned and sequenced the complete gag and env genes of 13 primary virus isolates. Phylogenetic analysis of our sequences and 69 complete env genes from the Los Alamos and GenBank databases revealed multiple subclusters within subtype C. The V3 loop region was relatively conserved in all our strains when compared with other subtypes, but the region immediately downstream was highly variable. No intersubtype recombinant forms were observed when comparing the gag and env sequences. Characterization of the complete gag and env genes enabled us to select specific strains for further vaccine development.en
dc.description.sponsorshipThis work was supported by grants from the Poliomyelitis Research Foundation (PRF), the South African AIDS Vaccine Initiative (SAAVI), and the Harry Crossley Foundation. Excellent technical assistance from Brenda Robson and Annette Laten is acknowledged. We also thank Thomas J. Scriba and Florette K. Treurnicht for helpful discussions and critical reading of the manuscripten
dc.format.extent579141 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationEngelbrecht, S, De Villiers, T, Sampson, CC, Zur Megede, J, Barnett, SW & Janse van Rensburg, E 2001,'Genetic analysis of the complete gag and env genes of HIV-1 subtype C primary isolates from South Africa', Aids Research and Human Retroviruses, vol. 17, no. 16, pp. 1533-1547. [http://www.liebertonline.com/aid?]en
dc.identifier.issn0889-2229
dc.identifier.other10.1089/08892220152644250
dc.identifier.urihttp://hdl.handle.net/2263/3217
dc.language.isoenen
dc.publisherMary Ann Lieberten
dc.rightsMary Ann Liebert.en
dc.subjectHIV-1en
dc.subject.lcshHIV (Viruses) -- Research -- South Africa
dc.subject.lcshAIDS vaccines -- South Africa
dc.subject.lcshHIV (Viruses) -- Genetic aspects
dc.subject.lcshDisease susceptibility -- Genetic aspects
dc.titleGenetic analysis of the complete gag and env genes of HIV type 1 subtype C primary isolates from South Africaen
dc.typeTexten

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