Title page for ETD etd-12192005-153837


Document Type Doctoral Thesis
Author Stoltz, Anton Carel
URN etd-12192005-153837
Document Title Immunological properties of mycolic acids, the major lipid cell wall component of Mycobacterium tuberculosis
Degree DPhil (Human Physiology)
Department Physiology
Supervisor
Advisor Name Title
Prof J A Verschoor Committee Chair
Keywords
  • mycolic acids
  • immunology
  • mice
  • Mycobacterium tuberculosis
Date 2002-04-01
Availability unrestricted
Abstract
The immunological effects of mycolic acids (MA) from Mycobacterium tuberculosis on mouse peritoneal macrophages were studied. MA was solubilbized using various carriers. Phagosome uptake and maturation (into late stage phagolysosomes) were compared using fluorescent markers and the confocal microscope. During assessment on the effects of MA on mouse macrophages, changes in morphology and activation of the macrophages were found. This indicated that the MA was immune reactive towards macrophages. The phenotype of cell that develops after in vivo loading with MA was characterized by using cell surface markers: it was found that MA-Ioaded macrophages developed into foam cells. Cell survival, proliferation and macrophage cytokine production were examined to characterize the foam-like cells. The effect of MA-induced foam-like cells on living Mycobacterium tuberculosis was evaluated and increased bactericidal activity was found. The roles of reactive oxygen and nitrogen intermediates via myeloperoxidase were also examined and a theoretical mechanism for the formation of foam cells proposed. The possible role of myeloperoxidase in activation of macrophages, foam cell formation and killing of Mycobacterium tuberculosis is discussed. It is postulated that a possible relationship might exist between tuberculosis and atherosclerosis that is facilitated by mycolic acids.

2002 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Stoltz, AC 2002, Immunological properties of mycolic acids, the major lipid cell wall component of Mycobacterium tuberculosis, DPhil thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-12192005-153837/ >

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