Document Type Master's Dissertation Author Kullmann, Anne URN etd-11302011-133415 Document Title Effects of xylazine, romifidine and detomidine on haematology, serum biochemistry and splenic size in horses Degree MSc Department Companion Animal Clinical Studies Supervisor
Advisor Name Title Dr E Rioja Garcia Co-Supervisor Dr M Sanz Supervisor Keywords
- colloid osmotic pressure
- plasma osmolality
- total serum protein
- packed cell volume
- splenic size
- alpha 2 adrenoceptor agonist
Date 2011-09-09 Availability unrestricted AbstractAlpha 2 agonists are frequently used in equine medicine. This study focused primarily on α2 agonist-induced changes in PCV and TSP. A secondary aim of this study was to investigate the effects of α2 agonist on selected serum biochemical parameters and splenic size in order to identify potential causes for the changes seen in PCV and TSP.
Four healthy adult mares were treated in a blinded, randomized, cross-over design with a single dose of xylazine (0.5 mg/kg), romifidine (0.04 mg/kg) or detomidine (0.01 mg/kg) intravenously, or detomidine (0.02 mg/kg) intramuscularly. A 1-week washout period was allowed between treatments. Haematology, TSP, COP, plasma osmolality, glucose, BUN, serum lactate, electrolytes, venous blood pH, ultrasonographic splenic size and degree of clinical sedation were evaluated at different time points post-injection and compared to baseline values.
All treatments induced similar clinical sedation in the mares. A significant change over time in PCV and TSP following each treatment was identified, with overall median (range) maximal reductions compared to baseline of 20.9% (12.9 - 27.3%) and 5.8% (3.0 - 10.3%), respectively. Additionally, changes over time were significant for RBC count, BUN, COP and Ca2+, which decreased; and glucose, plasma osmolality, Na+ and splenic size, which increased, when compared to baseline. There was no significant main effect of treatment on PCV, TSP or any other parameters measured except for glucose.
This study concluded that changes in PCV, TSP and other biochemical parameters induced by α2 agonists should be taken into consideration when assessing critically ill horses that received these drugs. There was evidence of splenic RBC sequestration as well as fluid shifts; therefore, the results suggest a multifactorial cause for the changes in PCV and TSP.
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Please cite as follows:
Kullmann, A 2011, Effects of xylazine, romifidine and detomidine on haematology, serum biochemistry and splenic size in horses, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-11302011-133415 / >
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