Title page for ETD etd-09172012-152036


Document Type Doctoral Thesis
Author Dvir, Eran
Email eran.dvir@up.ac.za
URN etd-09172012-152036
Document Title Biomarkers of neoplastic transformation in canine spirocercosis
Degree PhD
Department Companion Animal Clinical Studies
Supervisor
Advisor Name Title
Dr R J Mellanby Co-Supervisor
Prof J P Schoeman Supervisor
Keywords
  • inflammatory fibroblastic nodule
  • Spirocerca lupi
  • parasite-associated malignancy
  • oesophageal sarcoma
Date 2012-09-07
Availability unrestricted
Abstract
Spirocerca lupi is a nematode that infects the dog’s oesophagus and promotes the formation of an inflammatory fibroblastic nodule that progresses to sarcoma in approximately 25% of cases. Differentiating neoplastic from non-neoplastic cases ante-mortally is challenging and has major therapeutic and prognostic implications. More importantly, spirocercosis-associated oesophageal sarcoma is an excellent and under-utilized spontaneous model of parasite-associated malignancy and the pathogenesis of the neoplastic transformation is poorly understood.

The current study objective was to investigate potential clinical, clinicopathological, radiological and tissue biomarkers for the malignant transformation and an attempt to use these biomarkers to gain a deeper understanding of the pathogenesis of the neoplastic transformation. Our central hypothesis was that the parasite produces excretory product(s) which diverts the immune response from a T helper 1 (Th1) to Th2 cell response, typical of many nematode infections, and further to an immunoregulatory (immunosuppressive), FoxP3+ regulatory T cell-predominated response which then facilitates neoplastic transformation.

The following parameters were studied and compared between cases with non-neoplastic and neoplastic spirocercosis: clinical presentation, haematology, serum albumin and globulin, thoracic radiology, haematoxylin-eosin (H&E) histology, Immunohistochemistry for expression of vascular endothelial growth factor (VEGF)-A, fibroblast growth factor (FGF), platelet-derived growth factor (PDGF), MAC387 (myeloid cells), CD3 (T cells), Pax5 (B cells) and FoxP3 (T regulatory cells) and plasma cytokine concentrations including IL-2, IL-4, IL-6, IL-8, IL-10, IL-18, GM-CSF and MCP-1.

Hypertrophic osteopathy showed 100% specificity for neoplastic transformation but relatively poor sensitivity (40%). Female gender, anaemia, leukocytosis, thrombocytosis, spondylitis and bronchial displacement were significantly more common in neoplastic cases, but appeared in non-neoplastic cases as well. The H&E study revealed 2 stages in the non-neoplastic nodules: early inflammation, characterized by fibrocytes and abundant collagen, and a pre-neoplastic stage, characterized by activated fibroblasts and reduced collagen. The neoplastic cases were all sarcomas, primarily osteosarcoma with very aggressive features comparable to other appendicular osteosarcoma in the dog. The inflammation in spirocercosis is characterized by pockets of pus (MAC387+ cells) surrounded by organized lymphoid foci (CD3+ and to a lesser degree Pax5+ cells). There was no evidence of a local accumulation of FoxP3+ cells, unlike many previous studies which have reported an increase in Foxp3+ T cells in both malignancies and parasite infections. Interleukin-8 plasma concentration was higher in the neoplastic group compared to the non-neoplastic and the control groups. Interleukin-18 concentration was higher in the non-neoplastic group followed by the control group and finally the neoplastic group.

As with most similar studies, no ideal biomarker with high sensitivity and specificity was identified. However, if examined together, a panel of the biomarkers that were identified more commonly in the neoplastic cases should substantially increase the index of suspicion for neoplastic transformation in a diagnosed spirocercosis case. The inflammatory response showed features of increased myeloid (innate) response and lymphocytic response with pro-inflammatory cytokines. This was not our initial hypothesis and the question remains whether the response is secondary to the worm infection, or to a symbiotic bacterium that is carried by the worm. The role of such a reaction in neoplastic transformation remains to be elucidated.

© 2012 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Dvir, E 2012, Biomarkers of neoplastic transformation in canine spirocercosis, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-09172012-152036/ >

D12/9/166/ag

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