Title page for ETD etd-09142010-200331


Document Type Master's Dissertation
Author Yelani, Thembela
Email s24443027@tuks.co.za
URN etd-09142010-200331
Document Title Isolation and identification of poisonous triterpenoids from Elaeodendron croceum
Degree MSc
Department Plant Production and Soil Science
Supervisor
Advisor Name Title
Prof J J M Meyer Supervisor
Keywords
  • cytotoxicity
  • Elaeodendron croceum
  • triterpenes
Date 2010-04-28
Availability unrestricted
Abstract

Various plant species have been reported traditionally as well as in scientific literature for cytotoxicity against animal species. Isolation of several poisonous compounds from plant species has been reported previously. Elaeodendron croceum is a well-known poisonous plant species of which the poisonous compounds have not yet been isolated.

A phytochemical investigation of E. croceum leaves guided by cytotoxicity against Vero cells, led to the isolation of five known compounds; 20-hydroxy-20-epi-tingenone (1), tingenone (2), tingenine B (3), 11α-hydroxy-β-amyrin (4), and naringenin (5). Compounds 1 and 2 showed the highest toxicity against Vero cells (IC50: 2.651 nM and 8.233 μM respectively). Cytotoxicity of the isolated compounds against three human cancer cell lines, HeLa, MCF-7, and SNO was also determined. Compounds 1 and 2 again showed the highest cytotoxicity with IC50 values ranging between 2.478 0.427 μM. This is the first report on the isolation, identification, and in vitro evaluation of poisonous compounds from E. croceum.

Copyright 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Yelani, T 2009, Isolation and identification of poisonous triterpenoids from Elaeodendron croceum, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-09142010-200331/ >

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