Document Type Master's Dissertation Author Yelani, Thembela firstname.lastname@example.org URN etd-09142010-200331 Document Title Isolation and identification of poisonous triterpenoids from Elaeodendron croceum Degree MSc Department Plant Production and Soil Science Supervisor
Advisor Name Title Prof J J M Meyer Supervisor Keywords
- Elaeodendron croceum
Date 2010-04-28 Availability unrestricted Abstract
Various plant species have been reported traditionally as well as in scientific literature for cytotoxicity against animal species. Isolation of several poisonous compounds from plant species has been reported previously. Elaeodendron croceum is a well-known poisonous plant species of which the poisonous compounds have not yet been isolated.
A phytochemical investigation of E. croceum leaves guided by cytotoxicity against Vero cells, led to the isolation of five known compounds; 20-hydroxy-20-epi-tingenone (1), tingenone (2), tingenine B (3), 11α-hydroxy-β-amyrin (4), and naringenin (5). Compounds 1 and 2 showed the highest toxicity against Vero cells (IC50: 2.651 nM and 8.233 μM respectively). Cytotoxicity of the isolated compounds against three human cancer cell lines, HeLa, MCF-7, and SNO was also determined. Compounds 1 and 2 again showed the highest cytotoxicity with IC50 values ranging between 2.478 – 0.427 μM. This is the first report on the isolation, identification, and in vitro evaluation of poisonous compounds from E. croceum.
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Please cite as follows:
Yelani, T 2009, Isolation and identification of poisonous triterpenoids from Elaeodendron croceum, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-09142010-200331/ >
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