Document Type Doctoral Thesis Author Mngomba, Simon Alfred firstname.lastname@example.org URN etd-07302008-123544 Document Title Development of clonal propagation protocols for Uapaca kirkiana and Pappea capensis, two southern African trees with economic potential Degree PhD Department Plant Production and Soil Science Supervisor
Advisor Name Title Dr F K Akinnifesi Committee Co-Chair Dr H M Venter Committee Co-Chair Prof E S du Toit Supervisor Keywords
- Miombo woodland
- graft compatibility
- seed germination
Date 2007-09-06 Availability unrestricted Abstract
Experiments were carried out with the objectives of developing propagation protocols for Uapaca kirkiana and Pappea capensis tree species of southern Africa, and evaluating the graft compatibility within U. Kirkiana tree clones, provenances and species. Reverse phase high performance liquid chromatography (RP-HPLC), Folin-Ciocalteau reagent, fluorescence microscopy and callus fusion methodologies were used to diagnose graft compatibility. Results indicated that U. Kirkiana culture asepsis was achieved with 0.1% w/v mercuric chloride HgCl2) and using pre-conditioned grafted trees. Sodium hypochlorite (NaOCl) improved P. Capensis seed asepsis and germination, and discarding floating seeds improved germination. Murashige and Skoog (MS) medium with 2.0 mg l-1 benzylaminopurine (BAP) and 0.3 mg l-1 casein hydrolysate (CH) was superior in shoot multiplication and 0.5 mg l-1 indole-3-butyric acid (IBA) for rooting of P. Capensis microshoots. For somatic embryogenesis, three quarter strength MS medium with 0.05 mg l-1 thidiazuron (TDZ) and 0.3 mg l-1 CH, or 0.2 mg l-1 BAP with 0.3 mg l-1 CH, were effective in germination of P. Capensis somatic embryos.
For U. Kirkiana lateral shoot explants, shoot multiplication was superior on three quarter strength MS medium with 0.1 mg l-1 BAP and 0.3 mg l-1 CH. Rooting of micro-cuttings (36%) was achieved on ½ MS with 2.5 mg l-1 IBA. RP-HPLC, fluorescence microscopy and callus fusion studies showed that phenolic compounds play a major role in U. Kirkiana graft incompatibility. Less graft compatible combinations showed an increase in phenol deposits above the union and graft incompatibility was more pronounced above the union than below the union. Proliferation of parenchymatous tissues was better below the union than above the union. Fluorescence microscopy showed presence of flavonoids and polymers above the union of less graft compatible combinations. The chromatograms showed that ferulic acid was abundant and responsible for wood discolouration. The chromatograms also isolated ρara-coumaric acids which were predominant above the union of the less compatible combinations. Therefore, ρara-coumaric acids, flavonoids and polymers were implicated in graft incompatibility of U. kirkiana trees.
Copyright © 2007, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.
Please cite as follows:
Mngomba, SA 2007, Development of clonal propagation protocols for Uapaca kirkiana and Pappea capensis, two southern African trees with economic potential, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07302008-123544/ >
Filename Size Approximate Download Time (Hours:Minutes:Seconds)
28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access 00front.pdf 185.71 Kb 00:00:51 00:00:26 00:00:23 00:00:11 < 00:00:01 01chapter1.pdf 524.61 Kb 00:02:25 00:01:14 00:01:05 00:00:32 00:00:02 02chapter2.pdf 318.99 Kb 00:01:28 00:00:45 00:00:39 00:00:19 00:00:01 03chapter3.pdf 803.09 Kb 00:03:43 00:01:54 00:01:40 00:00:50 00:00:04 04chapter4.pdf 657.44 Kb 00:03:02 00:01:33 00:01:22 00:00:41 00:00:03 05chapter5.pdf 1.23 Mb 00:05:42 00:02:55 00:02:33 00:01:16 00:00:06 06chapter6.pdf 305.18 Kb 00:01:24 00:00:43 00:00:38 00:00:19 00:00:01 07chapter7.pdf 357.74 Kb 00:01:39 00:00:51 00:00:44 00:00:22 00:00:01 08chapter8.pdf 659.06 Kb 00:03:03 00:01:34 00:01:22 00:00:41 00:00:03 09chapter9.pdf 147.88 Kb 00:00:41 00:00:21 00:00:18 00:00:09 < 00:00:01 10references.pdf 539.17 Kb 00:02:29 00:01:17 00:01:07 00:00:33 00:00:02