Title page for ETD etd-07272010-110425


Document Type Master's Dissertation
Author De Wet, Tanya
URN etd-07272010-110425
Document Title Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro
Degree MChD
Department Periodontics and Oral Medicine
Supervisor
Advisor Name Title
Prof S J Botha Supervisor
Keywords
  • minocline and doxycycline concentrations
Date 2006-11-01
Availability unrestricted
Abstract
Minocycline (MC) and Doxycycline (DC) are used worldwide as locally applied adjuncts in the treatment of periodontal diseases. As a group the tetracyclines are well known for their advantageous properties. There is however possible cytotoxicity towards cells in the area of application.

This study determined the minimum cytocidal concentration of MC and DC on the growth and proliferation of Human Periodontal Ligament Fibroblasts (HPLF) in vitro. This was facilitated by growing cells (PDL1 and PDL2) in the presence of MC and DC in media in 96 tissue wells starting at a concentration of 1400 g.ml-1 (100%). Serial dilutions of the MC and DC at 10% increments were investigated in order to detect significant HPLF cell growth inhibition. The significant LD50 was further determined at one percent increments in order to arrive at a specific percentage value.

The results were read as LD50 values from growth concentration curves. The LD50 of MC on PDL1 and PDL2 after one hour exposure was 686 g.ml-1 and 896 g.ml-1 respectively while for DC it was 252 g.ml-1 and 546 g.ml-1. The LD50 of MC on PDL1 and PDL2 after 24 hour exposure was 196 g.ml-1 and 266 g.ml-1 respectively while for DC it was 252 g.ml-1 for both. The LD50 of MC on PDL1 and PDL2 after 48 hour exposure was 252 g.ml-1 and 182 g.ml-1 respectively while for DC it was 154 g.ml-1 and 168 g.ml-1.

Based upon the LD50 values this study found that DC is more cytotoxic than MC and linked to this, the two cell lines reacted slightly differently. The concentrations MC and DC tested in this study did however not influence growth of HPLF significantly.

2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

De Wet, T 2006, Minimum cytocidal effect of different minocycline and doxycycline concentrations to human periodontal ligament fibroblasts in vitro, MChD dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07272010-110425/ >

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