Title page for ETD etd-07232010-133809


Document Type Master's Dissertation
Author Heyman, Heino Martin
Email hmheyman@gmail.com
URN etd-07232010-133809
Document Title Metabolomic comparison of selected Helichrysum species to predict their antiviral properties
Degree MSc
Department Plant Science
Supervisor
Advisor Name Title
Prof J J M Meyer Committee Chair
Dr V Maharaj Committee Co-Chair
Keywords
  • nuclear magnetic resonance
  • NMR
  • PCA
  • H. adenocarpum subsp
  • adenocarpum
  • OPLS-DA
  • Discriminate Analysis
  • Helichrysum
  • Orthogonal Projection to Latent Structure
  • Helichrysum populifolium
  • Principal Component Analysis
  • metabolomics
Date 2010-04-28
Availability unrestricted
Abstract

From the Helichrysum genus 600 species occur in Africa of which 244 species are found in South Africa. The most commonly used Helichrysum species for medicinal purposes are H. cymosum, H. odoratissimum, H. petiolare and H. nudifolium. The medicinal uses include the treatment of coughs, colds, fever, infection, headaches, menstrual pain and are very popular for wound dressing. Previous published research has shown that H. aureonitens has antiviral properties against Herpes simplex virus type 1 (HSV-1). In this study, further investigation into the Helichrysum species was undertaken, to establish the active constituents responsible for anti-HSV activity using a metabolomics approach. The cytotoxicity of 12 Helichrysum species was investigated and ranged from <3.125 μg/ml to 277.8 μg/ml on the vero cell line. The 12 Helichrysum species also showed various levels of antiviral activity against HSV, with both the water-methanol and chloroform extracts of H. adenocarpum subsp. adenocarpum being the most active extract at 25 μg/ml.

In this study the activity of Helichrysum species against HIV-1 RT was also investigated. Helichrysum populifolium was the most active extract, inhibiting the HIV-1 RT enzyme by 63.78 % at 200 μg/ml. The bioactivity data and the spectral nuclear magnetic resonance (NMR) data of al the Helichrysum species from this study was analysed using the SIMCA-P software to discriminate between the different species on the basis of their bioactivity and chemical composition. The samples did not group well on Principal Component Analysis (PCA) but did separate well using the Orthogonal Projection to Latent Structure Discriminate Analysis (OPLS-DA) on the basis of their activity and NMR spectra data. From the OPLS scoring plots analysis, contribution plots were created which indicated regions responsible for the difference between the species, with these regions being investigated to identify the bioactive constituents. It was thus possible to use metabolomics to discriminate between samples on the basis of their activity and show that it could probably be used in future as a tool to identify active ingredients in medicinal plants and accelerate drug discovery.

Copyright 2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Heyman, HM 2009, Metabolomic comparison of selected Helichrysum species to predict their antiviral properties, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07232010-133809/ >

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