Title page for ETD etd-07152008-144250

Document Type Master's Dissertation
Author Koch, Orienka
URN etd-07152008-144250
Document Title Towards the diagnosis of two intracellular pathogens of grapevine in South Africa
Degree MSc (Microbiology)
Department Microbiology and Plant Pathology
Advisor Name Title
Prof G Pietersen Co-Supervisor
Prof L H Nel Supervisor
  • intracellular pathogens of grapevine
  • South Africa
Date 2006-09-07
Availability unrestricted
A survey was conducted, from 2001 to 2004, of viruses spreading within certified grapevine material in South Africa. As far as possible, viruses were identified and detection methods established. However, unknown spherical virus-like particles were observed in samples that also contained Grapevine Leafroll Associated Virus-Type 3. The unknown spherical particles were thought to most likely be Grapevine Fleck Virus, which was previously found in South Africa.

A PCR method to be used locally for the routine detection of Grapevine Fleck Virus was established and first used to determine whether any of the greenhouse and field samples with the unknown spherical viruses were infected with Grapevine Fleck Virus.

During the 2001 to 2004 survey, plants with leafroll and reddening symptoms unlike classical grapevine leafroll disease were also observed. No grapevine leafroll-associated viruses could be detected in these, but the symptoms observed resembled symptoms induced by phytoplasmas in Europe.

A PCR method for the routine universal detection of phytoplasmas was established and this method was used to determine if phytoplasmas were associated with the symptomatic plants found. Sequence information from PCR amplicons suggest the presence of Candidatus phytoplasma solani, found for the first time in South Africa. This important finding however requires conformation by a second laboratory.

2006 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Koch, O 2006, Towards the diagnosis of two intracellular pathogens of grapevine in South Africa, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07152008-144250/ >


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