Title page for ETD etd-07132009-174458


Document Type Master's Dissertation
Author Brothers, Peter Stanley
Email peter@brotherssafaris.com
URN etd-07132009-174458
Document Title Occurrence of blood-borne tick-transmitted parasites in tsessebe (Damaliscus lunatus lunatus) antelope in Vaalbos National Park, Northern Cape Province
Degree MSc
Department Veterinary Tropical Diseases
Supervisor
Advisor Name Title
Dr N E Collins Co-Supervisor
Prof B L Penzhorn Supervisor
Keywords
  • wildlife species
  • Vaalbos National Park
  • antelope
Date 2009-04-29
Availability unrestricted
Abstract

Blood was collected from 71 tsessebe antelope and ticks from 12 of these animals, in the Vaalbos National Park. The samples were collected when the animals were relocated to a new park as a result of the deproclamation of Vaalbos National Park. DNA was extracted from the blood samples and the V4 hypervariable region of the 18S rRNA gene of any piroplasm parasites present was amplified by PCR. The RLB hybridisation technique was used to identify piroplasm parasites. Theileria spp. were identified and full-length 18S rRNA genes were amplified, cloned and sequenced. The results indicated the presence of novel Theileria spp. phylogenetically very closely related to both Theileria sp. (sable) and Theileria separate. The ticks collected were all Rhipicephalus evertsi evertsi, which has been shown to be capable of acting as a vector for Theileria spp. All animals appeared to be in good health at the time of sampling and after relocation. It is thus possible that, as with several other wildlife species, the Theileria spp. identified here do not cause disease under normal circumstances and that a situation of endemic stability exists. Once the host is under any form of stress, however, overt clinical disease may well become evident. The significance of these Theileria spp. should not be underestimated, and care should be taken not to transmit the organisms into new areas. More research will need to be conducted to determine the exact clinical significance of these findings and the role of Rhipicephalus evertsi evertsi as a potential vector for these Theileria spp.

Copyright 2008, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Brother, PS 2008, Occurrence of blood-borne tick-transmitted parasites in tsessebe (Damaliscus lunatus lunatus) antelope in Vaalbos National Park, Northern Cape Province, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07132009-174458/ >

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