Title page for ETD etd-07082011-165915


Document Type Master's Dissertation
Author Pallant, Christopher Alexander
URN etd-07082011-165915
Document Title Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.)
Degree MSc
Department Pharmacology
Supervisor
Advisor Name Title
Dr A D Cromarty Co-Supervisor
Prof V Steenkamp Supervisor
Keywords
  • methicillin-resistant staphylococcus aureus
  • indole alkaloids
  • MRSA
  • antibacterial natural products
  • Tabernaemontana elegans
  • tuberculosis
Date 2011-04-08
Availability unrestricted
Abstract
Bacterial infections remain a significant threat to human health. Due to the emergence of widespread antibiotic resistance, development of novel antibiotics is required in order to ensure that effective treatment remains available. The aim of this study was to isolate and identify the fraction responsible for the antimicrobial activity in Tabernaemontana elegans (Stapf.) root extracts.

The active fraction was characterised by thin layer chromatography (TLC) and gas chromatography mass spectrometry (GC-MS). Antibacterial activity was determined using the broth micro-dilution assay and antimycobacterial activity using the BACTEC radiometric assay. Cytotoxicity of the crude extract and fractions was assessed against primary cell cultures; lymphocytes and fibroblasts; as well as a hepatocarcinoma (HepG2) and macrophage (THP-1) cell line using the Neutral Red uptake and MTT assays. The crude root extracts were found to contain a high concentration of alkaloids (1.2% w/w). GC-MS analysis identified the indole alkaloids, voacangine and dregamine, as major components. Antibacterial activity was limited to the Gram-positive bacteria and Mycobacterium species, with MIC values in the range of 64 256 g/ml. When combined with antibiotics, additive antibacterial effects were observed. Marked cytotoxicity to all cell lines tested was evident in the MTT and Neutral Red uptake assays, with IC50 values ranging between 1.11 9.81 g/ml.

This study confirms the antibacterial activity of T. elegans and supports its potential for being investigated further for the development of a novel antibacterial compound.

2010, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Pallant, CA 2010, Bioactivity of the alkaloidal fraction of Tabermaemintana elegans (Stapf.), MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-07082011-165915 / >

E11/304/gm

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  dissertation.pdf 1.76 Mb 00:08:07 00:04:10 00:03:39 00:01:49 00:00:09

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact UPeTD.