Title page for ETD etd-06082007-152035

Document Type Doctoral Thesis
Author Mekbib, Sissay Bekele
Email sisbekele@yahoo.com
URN etd-06082007-152035
Document Title Identification of citrus (Citrus sinensis) Postharvest Pathogens from Ethiopia and their Control
Degree PhD (Crop Protection)
Department Microbiology and Plant Pathology
Advisor Name Title
Prof L Korsten
  • Ethiopia
  • control
  • Identification of citrus (Citrus sinensis) posthar
Date 2007-04-20
Availability unrestricted
From a world prospective, the continuous application of chemical pesticides has serious long-term effects on human health and environmental pollution, and can result in resistant pathogen strains. However, postharvest diseases cause major losses on the markets and need to be controlled effectively. The search for biopesticides using microbial antagonists and natural plant products has subsequently become more important as viable alternatives to control postharvest diseases. Currently, little information exists in terms of citrus production practices, disease management measures and postharvest losses in Ethiopia. The aim of this study was therefore to determine what the current situation in the country is in terms of production, disease management and postharvest disease incidence, disease management practices in Ethiopia and to develop an effective and safe disease control strategy for the industry. Citrus production in Ethiopia is mainly done by Government enterprises with little technical expertise. Disease control strategies are ineffective with postharvest losses exceeding 46%. The most important postharvest pathogen identified was Penicillium digitatum. In development of biopesticides, three yeast antagonists [Cryptococcus laurentii (strain MeJtw 10-2 and strain TiL 4-3) and Candida sake (TiL 4-2)] and plant leaf extracts of Acacia seyal and Withania somnifera were found to have some potential to control Penicillium in in vitro and in vivo trials and ensure fruit quality. The modes of action of the yeast antagonists were not based on antibiosis. Instead, it involved competitive colonization where the antagonists inhibited P. digitatum spore germination and reduced mycelial growth by 75-100%. Extracts from the two plant species showed broad-spectrum antimicrobial activity against a range of several fungal and bacterial pathogens. The semi-commercial application of the antagonists and plant extracts improve fruit quality and the integration of these biopesticides were found effective in semi commercial trials and may provide a commercial solution for the citrus industry.

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  00front.pdf 33.97 Kb 00:00:09 00:00:04 00:00:04 00:00:02 < 00:00:01
  01chapter1.pdf 32.95 Kb 00:00:09 00:00:04 00:00:04 00:00:02 < 00:00:01
  02chapter2.pdf 274.96 Kb 00:01:16 00:00:39 00:00:34 00:00:17 00:00:01
  03chapter3.pdf 965.18 Kb 00:04:28 00:02:17 00:02:00 00:01:00 00:00:05
  04chapter4.pdf 419.89 Kb 00:01:56 00:00:59 00:00:52 00:00:26 00:00:02
  05chapter5.pdf 273.22 Kb 00:01:15 00:00:39 00:00:34 00:00:17 00:00:01
  06chapter6.pdf 336.44 Kb 00:01:33 00:00:48 00:00:42 00:00:21 00:00:01
  07chapters7-8.pdf 99.80 Kb 00:00:27 00:00:14 00:00:12 00:00:06 < 00:00:01
  08back.pdf 118.58 Kb 00:00:32 00:00:16 00:00:14 00:00:07 < 00:00:01

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