Document Type	Master's Dissertation
Author	Sibanda, Donald Ray
Email	donruz@yahoo.com
URN	etd-05242012-144516
Document Title	Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana
Degree	MSc
Department	Veterinary Tropical Diseases
Supervisor	Advisor Name Title Prof B L Penzhorn Co-Supervisor Dr P T Matjila Supervisor
Keywords	domestic dogs Botswana tick-borne pathogens
Date	2012-04-13
Availability	unrestricted
Abstract The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from dogs that underwent a spay and neuter program in Maun, Botswana. These were sent to the Department of Veterinary Tropical Diseases, University of Pretoria, and were stored at the Molecular Laboratory. DNA was extracted from the samples followed by the Polymerase Chain Reaction (PCR) and the RLB. Six samples were found to be positive for Ehrlichia canis. Two of the samples were positive for a concurrent infection of E. canis and Ehrlichia ruminantium. There was one sample that was positive for the non-pathogenic Ehrlichia sp. Omatjenne. Although six samples were positive for the Babesia/Theileria catch-all probe, none hybridised with any of the species-specific probes. Copyright © 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria Please cite as follows: Sibanda, D R 2011, Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-05242012-144516 / > E12/4/306/gm
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