Title page for ETD etd-05242012-144516


Document Type Master's Dissertation
Author Sibanda, Donald Ray
Email donruz@yahoo.com
URN etd-05242012-144516
Document Title Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana
Degree MSc
Department Veterinary Tropical Diseases
Supervisor
Advisor Name Title
Prof B L Penzhorn Co-Supervisor
Dr P T Matjila Supervisor
Keywords
  • domestic dogs
  • Botswana
  • tick-borne pathogens
Date 2012-04-13
Availability unrestricted
Abstract
The occurrence of tick-borne pathogens in dogs on communal land in Botswana was studied using Reverse Line Blot (RLB). Eighty blood samples were collected from dogs that underwent a spay and neuter program in Maun, Botswana. These were sent to the Department of Veterinary Tropical Diseases, University of Pretoria, and were stored at the Molecular Laboratory. DNA was extracted from the samples followed by the Polymerase Chain Reaction (PCR) and the RLB. Six samples were found to be positive for Ehrlichia canis. Two of the samples were positive for a concurrent infection of E. canis and Ehrlichia ruminantium. There was one sample that was positive for the non-pathogenic Ehrlichia sp. Omatjenne. Although six samples were positive for the Babesia/Theileria catch-all probe, none hybridised with any of the species-specific probes.

Copyright 2011, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria

Please cite as follows:

Sibanda, D R 2011, Molecular characterization of tick-borne pathogens of domestic dogs from communal areas in Botswana, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-05242012-144516 / >

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