Document Type Master's Dissertation Author Lentsoane, Robert URN etd-05232005-102711 Document Title Antimicrobial activity of Melianthus villosus Degree MSc (Botany) Department Botany Supervisor
Advisor Name Title Prof J J M Meyer Committee Chair Keywords
- antifungal agents
- anti-infective agents
- medicinal plants Africa Southern
- melianthus ethnobotany Africa Southern
- applied ethnobotany Africa Southern
Date 2008-02-17 Availability unrestricted AbstractMany South Africans continue to use traditional medicine in their daily lives as an alternative form of health care, also as part of their cultural heritage. Medicinal plants are proving to be an important source of novel drugs, and the knowledge provided by traditional healers is a useful tool in the search for antimicrobials.
The antimicrobial activity of
was investigated against ten bacteria and six fungi. The antibacterial assay showed that the root extract had the highest inhibition against the Gram-positive bacteria at the minimum inhibition concentration of o.1 mg/ml, as well as against the Gram-negative, E. coli, at the MIC of 1.0 mg/ml.
Antifungal activity was witnessed against Cladosporium cladosporoide, C. cucumerinum & C sphaerosperum all at the minimum inhibitory concentration of 1.0 mg/ml.
An attempt was made to isolate and identify the active antimicrobial compounds. A flavonol, quercetin was isolated and identified by means of UV spectral graphs, and TLC comparison of the plant extract and standard. However, a second isolated antibacterial compound could not be identified fully but it can be said that it is a triterpenoid.
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Please cite as follows:
Lentsoane, R 2004, Antimicrobial activity of Melianthus villosus, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-05232005-102711/ >
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