Title page for ETD etd-05152008-112158


Document Type Doctoral Thesis
Author Falzone, Nadia
URN etd-05152008-112158
Document Title The effect of non thermal 900 MHZ mobile phone radiation on human spermatozoa
Degree PhD (Reproductive Biology)
Department Obstetrics and Gynaecology
Supervisor
Advisor Name Title
Prof D R Franken Co-Supervisor
Prof Leszczynski Co-Supervisor
Dr C Huyser Supervisor
Keywords
  • apoptosis
  • human spermatozoa
  • mobile phone radiation
  • sperm functionality
  • stress response
Date 2007-11-23
Availability unrestricted
Abstract

Several studies have highlighted the possibility that radio-frequency electromagnetic fields (RF-EMF) used in mobile phone technology could influence DNA integrity of male germ cells as well as sperm motility. Current knowledge concerning the influence of RF-EMF on male germ cells is extremely limited. In the present study the hypothesis that 900 MHz GSM radiation could induce the activation of stress response in human spermatozoa was investigated.

Ejaculated, density purified, human spermatozoa from donors were exposed to 900 MHz GSM mobile phone radiation at specific absorption rate (SAR) levels of 2.0 and 5.7 W/kg and examined at various time points post exposure. Sperm motility and morphology were evaluated by computer-aided sperm analysis (CASA). The ability of RF-EMF exposed sperm to undergo the acrosome reaction was evaluated by flow cytometry. Sperm binding to the zona pellucida of human oocytes was determined by the hemi-zona (HZA) assay. Apoptotic markers, phosphatidylserine (PS) externalization, change in mitochondrial membrane potential (Δψm), reactive oxygen species (ROS) generation, caspase activation and DNA fragmentation were analysed using flow cytometry. Heat shock protein (Hsp) 27 and 70 expression and activity were analyzed using specific antibodies with flow cytometry and Western blot methods. Stress fibre stabilization (F-actin polymerization) was visualized using fluorescent dye labelled phalloidin.

No effect was seen on kinematic parameters assessed at SAR 2.0 W/kg, however straight line velocity (VSL) and beat cross frequency (BCF) were significantly altered after exposure at SAR 5.7 W/kg. Sperm shrinkage (decrease in surface area) was observed at both exposure levels. RF-EMF did not influence exposed spermatozoa’s ability to undergo the acrosome reaction. A significant decrease in sperm-zona binding was observed at both exposure levels. RF radiation did not have an effect on any apoptotic markers. ROS generation increased significantly with an increase in SAR (5.7 W/kg). RF-EMF did not induce a stress response in exposed sperm (no activation of Hsp70 and 27 activity).

These results cannot be ascribed to heating, as the temperature did not increase by more than 0.2 - 0.3ºC during exposure. The decrease in sperm-zona binding is the result of an alternative non-stress inducible pathway. This study should be replicated at lower SAR levels that would simulate the radiation absorption from carrying the cell phone in a pocket close to the testes.

© University of Pretoria, 2007

Files
  Filename       Size       Approximate Download Time (Hours:Minutes:Seconds) 
 
 28.8 Modem   56K Modem   ISDN (64 Kb)   ISDN (128 Kb)   Higher-speed Access 
  00front.pdf 133.59 Kb 00:00:37 00:00:19 00:00:16 00:00:08 < 00:00:01
  01sectionA.pdf 869.60 Kb 00:04:01 00:02:04 00:01:48 00:00:54 00:00:04
  02sectionB.pdf 2.49 Mb 00:11:32 00:05:56 00:05:11 00:02:35 00:00:13
  03sectionC.pdf 75.44 Kb 00:00:20 00:00:10 00:00:09 00:00:04 < 00:00:01
  04sectionD.pdf 547.35 Kb 00:02:32 00:01:18 00:01:08 00:00:34 00:00:02

Browse All Available ETDs by ( Author | Department )

If you have more questions or technical problems, please Contact UPeTD.