Title page for ETD etd-04292010-092056


Document Type Doctoral Thesis
Author Oberholzer, Hester Magdalena
Email nanette.oberholzer@up.ac.za
URN etd-04292010-092056
Document Title Evaluation of the anti-inflammatory properties of a complex homeopathic product Modul8® using the BALB/c murine asthmatic animal model
Degree PhD
Department Anatomy
Supervisor
Advisor Name Title
Prof E Pretorius Supervisor
Keywords
  • complex homeopathic product Modul8®anti-inflammatory properties
Date 2010-04-16
Availability restricted
Abstract
Modul8® is a complex homeopathic product which acts as an immunomodulator by enhancing the immunity of an individual in order to favour a particular immunological response. Asthma is a chronic inflammatory disease to which many different treatment approaches have been applied. The aim of the current study was to investigate the effect of this natural, homeopathic product on ultrastructural, immunological and haematological parameters in the treatment of asthma by using the BALB/c asthmatic animal model.

Mice have traditionally been used as experimental animals in studying human diseases and although there is no animal model that is an exact replica of the human disease, they have helped to understand some of the basic mechanisms involved as well as the development of new therapies. Since there is a correlation between allergic mice and human asthmatics, the BALB/c murine asthmatic animal model was implemented and used in the current study.

Implementation of the BALB/c murine asthmatic animal model involved the sensitization, nebulization and treatment of mice over a period of 41 days. Mice were randomly divided into four experimental groups including a control group, an asthmatic group receiving no treatment, an asthmatic group exposed to physiological comparable levels of Modul8® and lastly an asthmatic group exposed to hydrocortisone as positive control. The animals were also weighed on specific reference days throughout the study to determine if any significant changes exist between the experimental groups’ weights over the experimental period.

Haematological analysis revealed that Modul8® possesses the ability to alter the asthmatic profile of the white blood cell counts and stabilize it to levels comparable with the control group. In the ultrastructural analysis of platelets and fibrin networks it seemed that Modul8® stabilized platelet and fibrin morphology to a profile similar to that of control animals. Comparison of the ultrastructure of platelets and fibrin networks of the BALB/c asthmatic animals to that of human asthmatics revealed that this animal model adequately mimics the human disease on this specific level. Modul8® was also shown to activate mice peritoneal macrophages in vitro and this was linked to the important role of activated macrophages in the immune response. Histological and ultrastructural analysis of the lungs showed that Modul8® has the ability to stabilize the changes and remodelling caused in the lungs by the inflammatory process involved in asthma.

It is therefore concluded that this homeopathic immunomodulator has the ability to positively influence the chosen parameters of the inflammatory response involved in asthma and therefore might successfully be used as an adjuvant therapeutic approach to the known anti-asthmatic and anti-inflammatory treatments.

© 2009 University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Oberholzer, HM 2009, Evaluation of the anti-inflammatory properties of a complex homeopathic product Modul8® using the BALB/c murine asthmatic animal model, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-04292010-092056/ >

D10/260/ag

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