Title page for ETD etd-03122010-195432


Document Type Doctoral Thesis
Author Koorts, Alida Maria
Email akoorts@medic.up.ac.za
URN etd-03122010-195432
Document Title Expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron during chronic immune stimulation
Degree PhD
Department Physiology
Supervisor
Advisor Name Title
Prof M Viljoen Supervisor
Keywords
  • chronic immune stimulation
  • H-subunit
  • ferritin
  • isoferritins
  • iron
  • L-subunit
Date 2009-11-11
Availability unrestricted
Abstract
Ferritin is the major protein responsible for the sequestration, storage and release of intracellular iron. The ferritin protein shell exists as heteropolymers of various combinations of two types of subunits, the H-subunit and L-subunit, a phenomenon that gives rise to the existence of isoferritins. As the roles of the H-subunit and L-subunit differ in the mineralization process, the subunit composition of ferritin will influence the metabolic properties of the assembled ferritin molecules.

The primary aim of the present study was to quantitatively measure the expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron in patients with chronic T-helper cell type-1 immune stimulation. A second aim was to investigate the possible role that the expression of the H-subunit and L-subunit of ferritin may have in the establishment and maintenance of an iron transfer block.

The study subjects included 48 patients with chronic diseases from the Department of Internal Medicine, Kalafong Hospital and 10 patients with osteoarthritis, scheduled for hip replacement at the Department of Orthopaedics, Pretoria Academic Hospital. Bone marrow and blood samples were collected from each patient. The expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron was quantitatively evaluated by post-embedding immunolocalisation with immunogold transmission electron microscopy.

The patients were subdivided into groups with a predominantly T-helper cell type-1 immune reaction (pro-inflammatory) and normal immune status on the basis of C-reactive protein, neopterin and cytokines (INF-γ, TNF-α, Il-1β, Il-6, Il-12, Il-2, Il-8, GM-CSF, Il-4, Il-5, TGF-β and Il-10).

The study showed

up-regulation of the H-subunit of ferritin in the bone marrow macrophage in patients with chronic T-helper cell type-1 immune stimulation

no effects for chronic T-helper cell type-1 immune stimulation on the expression of the L-subunit of ferritin in the bone marrow macrophage

no effects for chronic T-helper cell type-1 immune stimulation on the expression of either the H-subunit or L-subunit of ferritin in cells of the bone marrow erythron

a 70% prevalence of iron transfer block in patients with chronic T-helper cell type-1 immune stimulation

up-regulation of the H-subunit of ferritin in the bone marrow macrophage in osteoarthritis patients who had normal T-helper cell type-1 immune activity, but significantly increased TGF-β levels

up-regulation of the H-subunit of ferritin in the patients with iron transfer block

iron availability loses its primary role in the establishment of the circulating red blood profile in conditions with chronic pro-inflammatory activity

indications that the H-subunit and L-subunit of ferritin may play a role in the iron availability for red blood cell haemoglobin production

various correlations in the osteoarthritis patients between the H-subunit and L-subunit of ferritin and different cytokines

2009, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Koorts, AM 2009, Expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron during chronic immune stimulation, PhD thesis, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-03122010-195432/ >

D10/118/ag

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