Document Type Master's Dissertation Author Holo, Luxolo URN etd-03082010-172629 Document Title Enantioselective, potentiometric membrane electrodes for enantioanalysis of amino acids of clinical and pharmaceutical importance Degree MSc Department Chemistry Supervisor
Advisor Name Title Prof R I Stefan Supervisor Keywords
- potentiometric membrane electrodes
- chromatographic techniques
Date 2006-04-24 Availability unrestricted Abstract
The enantioanalysis of compounds of clinical and pharmaceutical became increasingly important because the enantiomers of the same substance may be markers for different disease or are having a different pathway in the body. The utilization of enantioselective, potentiometric membrane electrodes made the assay of a single enantiomer easier and faster. Also the reliability of the analytical information is higher than that obtained using chromatographic techniques.
The proposed electrodes are made by mixing graphite powder with paraffin oil to give carbon paste, which is modified by the addition of a chiral selector (e.g., cylodextrins, maltodextrins, macrocyclic antibiotics and fullerenes). This design is reliable.
The high sensitivity, selectivity, enantioselectivity, accuracy and precision made them suitable to be used for the enantioanalysis of different compounds of clinical and pharmaceutical importance (e.g., L-histidine, L-cysteine and R-clenbuterol) in pharmaceutical tablets, and/or serum and urine samples.
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Please cite as follows:
Holo, L 2005, Enantioselective, potentiometric membrane electrodes for enantioanalysis of amino acids of clinical and pharmaceutical importance, MSc dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-03082010-172629/ >
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