Title page for ETD etd-02242010-133045


Document Type Master's Dissertation
Author Yusufmia, Saira Banu Abdul Sattar
URN etd-02242010-133045
Document Title The prevalence of Theileria species infections in cattle at the edge of the Hluhluwe-iMfolozi Park, KwaZulu-Natal, South AFrica
Degree MSc (Veterinary Tropical Diseases)
Department Veterinary Tropical Diseases
Supervisor
Advisor Name Title
Dr N E Collins Co-Supervisor
Prof B L Penzhorn Supervisor
Keywords
  • Theileria spices
  • KwaZulu-Natal
  • South Africa
  • infections
  • cattle
  • buffy-coat
Date 2009-11-27
Availability restricted
Abstract

A number of theilerial parasites have been identified in cattle. The most important in sub-Saharan Africa is Theileria parva, which causes widespread mortality and morbidity in endemic areas. A survey was conducted using buffy-coat samples from 60 apparently healthy adult communal cattle (Nguni breed) herded at the edge of the Hluhluwe-iMfolozi Park to determine the prevalence of Theileria species using PCR and RLB. The simultaneous detection of Babesia species was carried out using the same blot. Determining the presence of Trypanosoma species was carried out using PCR-RFLP to determine their contribution to the overall disease burden, if any, in these cattle. Results showed that 5% of the samples were positive for T. parva. This significant finding along with that of Thompson et al. (2008), suggests that cattle in South Africa may also be subclinical carriers of T. parva parasites and not only buffaloes, as was previously believed. The laws regarding control of the parasite in South Africa may thus need to be revised. Other theilerial species identified in the samples were T. mutans, T. velifera and Theileria sp. (sable) of which the first two are normally found in cattle and are non-pathogenic. Theileria sp. (sable) is not normally found in cattle and its presence could be due to contamination at the DNA extraction stage. A small percentage of the samples was positive for B. bovis and B. bigemina; whole-blood specimens would probably have yielded higher prevalences. One sample was positive for B. rossi, which could have been a contamination or may suggest an accidental transmission, as it is normally only found in dogs. Only one of the samples was found to be positive for Trypanosoma vivax, and two for T. theileri, of which only the first species is pathogenic. It therefore also did not seem that trypanosomosis contributes significantly to the overall disease burden in this area. The focus in this study was on the prevalence of Theileria species in cattle from the Hluhluwe-iMfolozi area, with an emphasis on T. parva in particular.

Copyright 2008, University of Pretoria. All rights reserved. The copyright in this work vests in the University of Pretoria. No part of this work may be reproduced or transmitted in any form or by any means, without the prior written permission of the University of Pretoria.

Please cite as follows:

Yusufmia, SBAS 2008, The prevalence of Theileria species infections in cattle at the edge of the Hluhluwe-iMfolozi Park, KwaZulu-Natal, South AFrica, MSc (Veterinary Tropical Diseases) dissertation, University of Pretoria, Pretoria, viewed yymmdd < http://upetd.up.ac.za/thesis/available/etd-02242010-133045/ >

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