Document Type Doctoral Thesis Author Kritzinger, Quenton URN etd-02222006-103447 Document Title Antimicrobial activity and fumonisins associated with cowpea (Vigna unguiculata) Degree PhD Department Microbiology and Plant Pathology Supervisor
Advisor Name Title Prof T A S Aveling Keywords
- Fusarium proliferatum
- Vigna unguiculata
Date 2004-04-08 Availability unrestricted AbstractA survey involving 71 farmers from rural communities in Mpumalanga, South Africa was conducted to gather information regarding the importance and utilisation of cowpea. Cowpea was rated third most important in contributing to household security, preceded by maize and vegetable production. Cowpea was mainly produced for own consumption, as a source of income and as fodder for livestock to a lesser extent. The crop was used by 8.5% of the farmers for medicinal purposes. Results indicated that 20% of the farmers encountered problems with mouldiness during storage, with insect infestation to a lesser degree.
Cowpea seed samples from South Africa and Benin, West Africa were analysed for seed mycoflora and various fungal genera, particularly Aspergillus, Phoma and Lasiodiplodia were recorded. The results indicated an array of Fusarium spp. including F. equiseti, F. chlamydosporum, F. graminearum, F. proliferatum, F. sambucinum, F. scirpi, F. semitectum and F. subglutinans. The seed samples and the F. proliferatum isolates, cultured on maize patty media, were analysed for fumonisin production. Samples were extracted with methanol/water (70:30) and cleaned-up on strong anion exchange solid phase extraction cartridges. High-performance liquid chromatography with pre-column derivatisation using o-phthaldialdehyde was used for the detection and quantification of FB1, FB2 and FB3. The cowpea cultivars from South Africa had levels of FB1 ranging between 0.12 – 0.61 µg/g. All the F. proliferatum isolates produced FB1, FB2 and FB3 with total fumonisin concentration levels between 0.80 - 25.30 µg/g. The highest level of FB1 detected was 16.86 µg/g.
Surface-disinfected seeds were imbibed in sterile distilled water amended with FB1 to yield final concentrations of 10, 25, 50 and 100 ppm. Percentage germination was determined in paper towels according to the International Seed Testing Association (ISTA) rules. Root and shoot length was measured after 9 days. All the toxin concentrations significantly decreased seed germination whilst root and shoot elongation was inhibited by the 50 and 100 ppm concentrations. Embryonic seed tissue treated with FB1 indicated compaction of the protoplasm and separation of the plasma membrane from the cell wall. Lipid bodies accumulated and seemed to line the cell wall.
Acetone and ethanol extracts of the leaves of two cowpea cultivars exhibited significant inhibition of the growth of fungal plant pathogens at 5.0 mg/ml, with the exception of Fusarium equiseti. The growth of some fungi, in particular Alternaria alternate, was also reduced by lower concentrations of certain extracts. Acetone extracts of the Bechwana White cultivar inhibited growth of Staphylococcus aureus and Enterococcus faecalis at 2.5 mg/ml and Bacillus cereus, B. subtilis and Enterobacter cloacae at 5.0 mg/ml. Ethanol extracts of the same cultivar showed antibacterial activity against E. faecalis and E. cloacae at 5.0 mg/ml.
This study represents the first report on the natural occurrence of fumonisins on cowpea seed and the potential of F. proliferatum isolates from cowpea seed to produce fumonisins. The phytotoxic effects of FB1 on cowpea seeds as well as the antimicrobial potential of cowpea leaf extracts were demonstrated for the first time.
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28.8 Modem 56K Modem ISDN (64 Kb) ISDN (128 Kb) Higher-speed Access 00front.pdf 107.38 Kb 00:00:29 00:00:15 00:00:13 00:00:06 < 00:00:01 01chapter1.pdf 103.47 Kb 00:00:28 00:00:14 00:00:12 00:00:06 < 00:00:01 02chapter2.pdf 246.15 Kb 00:01:08 00:00:35 00:00:30 00:00:15 00:00:01 03chapter3.pdf 3.99 Mb 00:18:28 00:09:30 00:08:18 00:04:09 00:00:21 04chapter4.pdf 154.47 Kb 00:00:42 00:00:22 00:00:19 00:00:09 < 00:00:01 05chapter5.pdf 4.34 Mb 00:20:04 00:10:19 00:09:01 00:04:30 00:00:23 06chapter6.pdf 166.06 Kb 00:00:46 00:00:23 00:00:20 00:00:10 < 00:00:01 07chapter7.pdf 99.24 Kb 00:00:27 00:00:14 00:00:12 00:00:06 < 00:00:01 08appendices.pdf 106.72 Kb 00:00:29 00:00:15 00:00:13 00:00:06 < 00:00:01